Journal: International Journal of Molecular Sciences
Article Title: DNA Demethylation Induces Tree Peony Flowering with a Low Deformity Rate Compared to Gibberellin by Inducing PsFT Expression under Forcing Culture Conditions
doi: 10.3390/ijms23126632
Figure Lengend Snippet: Effect of 5-azaC and GA treatments on DNA methylation levels in the promoter regions of flowering-pathway-related genes and photosynthetic analysis. ( A ) Related expression of DNA methylation regulation genes PsDRM , PsCMT, and PsMET by RT-qPCR (Duncan’s test at p < 0.05, error bars indicate the ±SD and different letters for each day indicate significant differences, n = 3). ( B ) DNA methylation level in the promoter of flowering-pathway-related genes determined by McrBC-PCR; + and − indicate with and without GTP, respectively, in the digestion solution ( n = 3, representative results shown here). ( C ) DNA methylation in the promoter of PsFT based on MassARRAY (Duncan’s test at p < 0.05, error bars indicate the ±SD and n = 3). ( D ) CG context prediction 3 kb before the ATG site in the PsFT promoter and the cis -element prediction in the tested region. The demethylation level in the above CGs 1–2 and 3–5 are also shown here. ( E ) Morphological levels used for photosynthetic determination. ( F ) Analysis of net photosynthetic rate (Pn), stomatic conductance (Gs), intercellular CO 2 concentration (Ci), and transpiration rate (Tr) in the third leaf of each plant at 28 d. (Duncan’s test at p < 0.05 after analysis of variance; data are shown as the mean ±SD, and the different letters indicates significant differences among different treatments, n = 10).
Article Snippet: Quantitative methylation analysis of bisulfite-treated genomic DNA was conducted through Agena’s MassARRAY EpiTYPER system (Agena Bioscience, San Diego, CA, USA).
Techniques: DNA Methylation Assay, Expressing, Quantitative RT-PCR, Concentration Assay